Genipin and insulin combined treatment improves implant osseointegration in type 2 diabetic rats

Abstract Background Type 2 diabetes mellitus (T2DM) has a harmful effect on the stability and osseointegration of dental implants. T2DM induces mitochondrial damage by inhibiting AMPK signaling, resulting in oxidative stress poor osteogenesis peri-implant bone area. Genipin is major component gardenia fruits with strong antioxidant, anti-inflammation, antidiabetic actions, it also can activate quality control via pathway. The purpose this study was to investigate effects genipin insulin treatment implant rats explore underlying mechanisms. Methods Streptozotocin-induced diabetic received surgery their femurs were then assigned five groups that subjected different treatments for three months: group, insulin-treated group (10 IU/kg), genipin-treated (50 mg/kg), combination-treated group. Then, we regularly assessed weight glucose levels animals. Rats euthanized at 3 months after implantation procedure, femora harvested microscopic computerized tomography analysis, biomechanical tests, histomorphometric assessment. Results results indicated highest blood measured poorest osseointegration. mitigated hyperglycemia normalized, reactivated alleviated as well reversed negative There beneficial changes observed T2DM-genipin T2DM-insulin groups, but these less comparison combination Conclusion Our suggests could be an effective method promoting rats, which may related signaling.